Table 8. Relative gene expression in the brain after 2 h of acute thermal stress at 30°C

Recovery time (h)	hsp70	irf3	gpx	cox	ampkβ	pparα	pparγ	g6pase
0	1.00 ± 0.05^a	1.00 ± 0.00^b	1.00 ± 0.06	1.00 ± 0.24^b	1.00 ± 0.36	1.00 ± 0.30	1.00 ± 0.18^b	1.00 ± 0.17^b
2	0.53 ± 0.19^b	2.93 ± 0.85^ab	2.55 ± 0.27	0.99 ± 0.28^b	2.83 ± 0.94	1.70 ± 0.48	2.73 ± 0.36^ab	10.7 ± 4.5^ab
4	0.11 ± 0.02^c	2.23 ± 0.11^ab	4.93 ± 0.18	1.28 ± 0.41^b	2.37 ± 0.24	1.80 ± 0.28	5.64 ± 1.15^a	9.83 ± 4.19^ab
6	0.04 ± 0.01^c	3.14 ± 0.91^ab	2.74 ± 0.72	1.18 ± 0.49^b	1.55 ± 0.53	1.99 ± 0.57	2.32 ± 0.43^ab	13.0 ± 3.4^ab
12	0.01 ± 0.00^c	2.72 ± 0.24^ab	2.11 ± 0.25	1.4 ± 0.56^b	1.64 ± 1.26	1.10 ± 0.47	1.9 ± 0.17^ab	2.39 ± 0.72^b
24	0.01 ± 0.00^c	5.18 ± 1.39^a	6.03 ± 0.37	2.32 ± 0.32^ab	3.78 ± 0.95	1.83 ± 0.21	4.12 ± 0.54^ab	13.5 ± 4.5^ab
48	0.00 ± 0.00^c	4.49 ± 0.55^ab	6.79 ± 0.69	3.85 ± 1.14^a	3.85 ± 0.73	1.94 ± 0.21	5.08 ± 1.11^ab	22.6 ± 5.4^a
p-value	< 0.0001	0.0054	0.0861	0.0007	0.2077	0.8197	0.0024	< 0.0001

Values are means from triplicate groups of juvenile olive flounder where the values in each row with different superscripts are significantly different (mean ± SEM; p < 0.05).
Relative gene expression level (recovery time at 0 h was set as a control; 18s used as housekeeping gene).
hsp70, heat shock protein 70; irf3, interferon regulatory factor 3; gpx, glutathione peroxidase; cox, cytochrome c oxidase; ampkβ, adenosine-activated protein kinase beta; pparα, peroxisome proliferator-activated receptors alpha; pparγ, peroxisome proliferator-activated receptors gamma; g6pase, glucose-6-phosphatase.