Table 7. Relative gene expression in the liver after 6 h of acute thermal stress at 30°C

Recovery time (h)	hsp70	irf3	gpx	cox	ampkβ	pparα	pparγ	g6pase
0	1.00 ± 0.08^a	1.00 ± 0.49^b	1.00 ± 0.19^bc	1.00 ± 0.14^a	1.00 ± 0.25^a	1.00 ± 0.38^b	1.00 ± 0.16	1.00 ± 0.23^cd
2	0.15 ± 0.09^b	0.53 ± 0.35^b	2.45 ± 0.02^a	0.24 ± 0.08^b	0.08 ± 0.02^b	1.31 ± 0.46^b	0.92 ± 0.32	2.58 ± 0.72^a
4	0.02 ± 0.00^c	1.27 ± 0.39^b	1.62 ± 0.49^b	0.47 ± 0.17^b	0.06 ± 0.02^b	0.97 ± 0.87^b	1.11 ± 0.31	0.56 ± 0.14^d
6	0.00 ± 0.00^c	6.08 ± 6.20^a	0.96 ± 0.30^bc	0.45 ± 0.1^1b	0.13 ± 0.04^b	0.62 ± 0.41^b	0.95 ± 0.20	0.62 ± 0.22^d
12	0.00 ± 0.00^c	0.88 ± 0.44^b	0.76 ± 0.38^c	0.50 ± 0.19^b	0.13 ± 0.03^b	1.18 ± 0.44^b	0.80 ± 0.29	0.80 ± 0.25^cd
24	0.00 ± 0.00^c	2.34 ± 0.71^b	1.15 ± 0.15^bc	0.50 ± 0.21^b	0.17 ± 0.06^b	4.38 ± 2.18^a	0.78 ± 0.17	1.38 ± 0.28^bc
48	0.00 ± 0.00^c	1.54 ± 0.62^b	1.19 ± 0.20^bc	0.68 ± 0.29^ab	0.12 ± 0.04^b	5.45 ± 2.21^a	1.07 ± 0.25	1.83 ± 0.59^b
p-value	< 0.0001	< 0.0001	0.0086	0.0163	< 0.0001	< 0.0001	0.2562	0.0173

Values are means from triplicate groups of juvenile olive flounder where the values in each row with different superscripts are significantly different (mean ± SEM; p < 0.05).
Relative gene expression level (recovery time at 0 h was set as a control; 18s used as housekeeping gene).
hsp70, heat shock protein 70; irf3, interferon regulatory factor 3; gpx, glutathione peroxidase; cox, cytochrome c oxidase; ampkβ, adenosine-activated protein kinase beta; pparα, peroxisome proliferator-activated receptors alpha; pparγ, peroxisome proliferator-activated receptors gamma; g6pase, glucose-6-phosphatase.