Table 4. Plasma metabolites and stress responses in juvenile olive flounder exposed to acute thermal stress at 30°C for 6 h

Recovery time (h)	Hematocrit	GOT (U/L)	GPT (U/L)	TP (g/L)	TG (mg/dL)	TCHO (mg/dL)	GLU (mg/dL)	Cortisol¹⁾
0	20.5 ± 0.6	281 ± 81^a	46.5 ± 20.5^a	3.15 ± 0.09	438 ± 133	122 ± 7^a	20.3 ± 1.4^a	10.1 ± 0.86^a
2	17.8 ± 1.1	60.0 ± 9.6^b	37.5 ± 0.9^a	3.10 ± 0.12	331 ± 173	105 ± 8^ab	9.75 ± 1.44^b	8.35 ± 0.9^ab
4	16.0 ± 0.4	52.5 ± 6.3^b	28.5 ± 1.5^a	2.85 ± 0.19	323 ± 41	101 ± 5^ab	9.00 ± 0.00^b	7.51 ± 1.06^b
6	16.0 ± 1.6	48.8 ± 6.9^b	27.0 ± 2.1^a	2.10 ± 0.27	270 ± 56	93 ± 3.2^b	12.8 ± 1.4^b	8.18 ± 1.49^b
12	15.5 ± 1.0	46.5 ± 10.6^b	28.5 ± 2.9^a	3.00 ± 0.32	202 ± 46	106 ± 13^ab	13.5 ± 2.6^b	9.59 ± 2.97^c
24	18.3 ± 1.3	52.5 ± 3.6^b	33.0 ± 7.3^a	2.78 ± 0.19	347 ± 64	107 ± 14^ab	11.3 ± 2.3^b	7.87 ± 1.04^c
48	16.0 ± 0.4	40.5 ± 2.9^b	29.3 ± 2.6^a	2.78 ± 0.08	206 ± 86	105 ± 6^ab	11.3 ± 0.8^b	8.20 ±3.40^c
p-value	0.2879	< 0.0001	< 0.0001	0.0559	0.0510	0.0594	0.0140	0.0020

Values are means from triplicate groups of juvenile olive flounder where the values in each row with different superscripts are significantly different (mean ± SEM; p < 0.05).
Tested by Elisa kit (CUSABIO, Houston, TX, USA).
GOT, glutamic oxaloacetic transaminase; GPT, glutamic pyruvate transaminase; TP, total protein; TG, triglyceride; TCHO, total cholesterol; GLU, glucose.