Table 3. Plasma metabolites and stress responses in juvenile olive flounder exposed to acute th ermal stress at 30°C for 4 h

Recovery time (h)	Hematocrit	GOT (U/L)	GPT (U/L)	TP (g/L)	TG (mg/dL)	TCHO (mg/dL)	GLU (mg/dL)	Cortisol¹⁾
0	21.8 ± 1.3	148 ± 64^a	62.0 ± 21.2^a	3.68 ± 0.28	351 ± 103	117 ± 14	20.8 ± 2.1a	11.0 ± 2.5^a
2	17.5 ± 0.9	85.5 ± 27.5^ab	32.3 ± 9.3^b	3.00 ± 0.17	309 ± 91	103 ± 7	11.3 ± 0.8b	6.34 ± 0.73^b
4	16.8 ± 1.7	63.8 ± 10.7^b	34.5 ± 6.2^ab	3.08 ± 0.31	362 ± 114	111 ± 16	11.3 ± 0.8b	11.4 ± 3.3^b
6	14.0 ± 2.0	58.5 ± 9.9^b	30.0 ± 3.2^b	2.85 ± 0.31	293 ± 63	103 ± 7	11.3 ± 0.8b	7.55 ± 1.11^b
12	13.5 ± 0.3	36.8 ± 1.9^b	29.3 ± 0.8^b	1.73 ± 0.14	215 ± 89	92 ± 15	9.00 ± 1.22^b	5.79 ± 0.31^c
24	18.0 ± 0.4	41.3 ± 5.7^b	22.5 ± 1.9^b	3.38 ± 0.28	341 ± 106	120 ± 14	13.5 ± 0.9b	8.29 ± 1.07^c
48	15.0 ± 0.7	45.8 ± 8.3^b	36.8 ± 5.0^ab	2.55 ± 0.26	158 ± 42	90.8 ± 10	10.5 ± 2.6b	7.66 ± 1.24^c
p-value	0.6534	< 0.0001	< 0.0001	0.4277	0.1926	0.4402	0.0012	0.0040

Values are means from triplicate groups of juvenile olive flounder where the values in each row with different superscripts are significantly different (mean ± SEM; p < 0.05).
Tested by Elisa kit (CUSABIO, Houston, TX, USA).
GOT, glutamic oxaloacetic transaminase; GPT, glutamic pyruvate transaminase; TP, total protein; TG, triglyceride; TCHO, total cholesterol; GLU, glucose.