Table 2. Plasma metabolites and stress responses in juvenile olive flounder exposed to acute thermal stress at 30°C for 2 h

Recovery time (h)	Hematocrit	GOT (U/L)	GPT (U/L)	TP (g/L)	TG (mg/dL)	TCHO (mg/dL)	GLU (mg/dL)	Cortisol (ng/mL)1)
0	20.0 ± 2.4	114 ± 32a	58.5 ± 11.1a	2.93 ± 0.19	340 ± 132	95.3 ± 8.6	36.0 ± 6.4a	11.4 ± 1.2a
2	18.8 ± 0.3	59.3 ± 6.1bc	39.8 ± 0.8b	3.45 ± 0.09	433 ± 115	116 ± 8	14.3 ± 1.4b	7.52 ± 1.06ab
4	18.1 ± 1.1	83.3 ± 5.8ab	36.8 ± 7.6 b	3.45 ± 0.19	320 ± 107	116 ± 11	6.00 ± 0.00b	7.42 ± 0.79b
6	19.5 ± 0.7	76.5 ± 7.5abc	38.3 ± 5.8b	3.15 ± 0.19	439 ± 78	124 ± 4	15.0 ± 4.1b	7.48 ± 1.02b
12	17.3 ± 0.5	38.3 ± 1.9c	26.3 ± 1.4b	2.70 ± 0.12	230 ± 34	104 ± 8	12.8 ± 1.4b	0.05 ± 0.06c
24	16.8 ± 1.3	39.8 ± 5.3bc	29.3 ± 4.3b	2.85 ± 0.09	230 ± 43	113 ± 3	13.5 ± 1.5b	0.04 ± 0.05c
48	16.3 ± 2.8	66.0 ± 10.5bc	26.3 ± 6.3b	2.70 ± 0.41	255 ± 58	104 ± 6	11.3 ± 1.9b	0.12 ± 0.09c
p-value	0.0592	0.0003	0.0010	0.0517	0.0521	0.8463	< 0.0001	0.0019

Values are means from triplicate groups of juvenile olive flounder where the values in each row with different superscripts are significantly different (mean ± SEM; p < 0.05).
Tested by Elisa kit (CUSABIO, Houston, TX, USA).
GOT, glutamic oxaloacetic transaminase; GPT, glutamic pyruvate transaminase; TP, total protein; TG, triglyceride; TCHO, total cholesterol; GLU, glucose.