Table 10. Relative gene expression in the brain after 6 h of acute thermal stress at 30°C

Recovery time (h)	hsp70	irf3	gpx	cox	ampkβ	pparα	pparγ	g6pase
0	1.00 ± 0.07^a	1.00 ± 0.75^b	1.00 ± 0.35	1.00 ± 0.07^ab	1.00 ± 0.14	1.00 ± 0.34^a	1.00 ± 0.19^ab	1.00 ± 0.2
2	0.47 ± 0.15^ab	1.48 ± 0.53^b	1.14 ± 0.26	0.40 ± 0.07^bc	0.84 ± 0.13	0.72 ± 0.05^ab	1.93 ± 0.61^a	6.80 ± 2.52
4	0.10 ± 0.04^b	2.06 ± 0.74^ab	1.46 ± 0.28	0.39 ± 0.11^bc	0.6 ± 0.13	0.58 ± 0.18^ab	1.08 ± 0.26^ab	2.04 ± 0.43
6	0.02 ± 0.01^b	0.93 ± 0.36^b	1.88 ± 0.43	0.24 ± 0.11^c	0.49 ± 0.11	0.43 ± 0.07^b	0.70 ± 0.30^b	3.88 ± 1.03
12	0.01 ± 0.00^b	0.87 ± 0.24^b	0.74 ± 0.18	1.13 ± 0.22^a	0.47 ± 0.02	0.39 ± 0.07^b	0.48 ± 0.11^b	1.17 ± 0.10
24	0.01 ± 0.01^b	1.98 ± 0.95^ab	1.40 ± 0.13	0.45 ± 0.03^bc	0.60 ± 0.11	0.51 ± 0.05^ab	0.80 ± 0.23^b	4.49 ± 0.89
48	0.00 ± 0.00^b	3.24 ± 0.75^a	1.88 ± 0.60	0.47 ± 0.16^bc	0.67 ± 0.09	0.85 ± 0.18^ab	1.10 ± 0.25^ab	6.92 ± 0.41
p-value	< 0.0001	0.0304	0.4012	0.0274	0.0601	0.0101	0.0015	0.0504

Values are means from triplicate groups of juvenile olive flounder where the values in each row with different superscripts are significantly different (mean ± SEM; p < 0.05).
Relative gene expression level (recovery time at 0 h was set as a control; 18s used as housekeeping gene).
hsp70, heat shock protein 70; irf3, interferon regulatory factor 3; gpx, glutathione peroxidase; cox, cytochrome c oxidase; ampkβ, adenosine-activated protein kinase beta; pparα, peroxisome proliferator-activated receptors alpha; pparγ, peroxisome proliferator-activated receptors gamma; g6pase, glucose-6-phosphatase.