Table 8. Effects of temperature stress and taurine supplementation on plasma metabolites of juvenie olive flounder followed by the acute temperature exposure

Temperature (°C)	Diet	GOT (U/L)	GPT (U/L)	GLU (mg/dL)	TG (mg/dL)	TP (g/dL)	TCHO (mg/dL)
Interactive effect between the temperature stress and diet
19.5	Tau0.13	18.3 ± 3.93	14.3 ± 1.20	15.0 ± 1.7cd	480 ± 17	3.17 ± 0.27	166 ± 8
	Tau0.56	14.0 ± 0.0	12.7 ± 0.3	13.3 ± 0.3d	307 ± 3	3.30 ± 0.15	174 ± 11
	Tau0.94	13.7 ± 0.9	11.7 ± 1.5	13.7 ± 0.3d	346 ± 26	3.10 ± 0.00	156 ± 5
	Tau1.50	19.3 ± 3.0	14.3 ± 2.6	13.3 ± 0.3d	326 ± 69	3.30 ± 0.06	175 ± 2
	Tau2.02	13.7 ± 1.2	12.7 ± 1.2	13.0 ± 0.6d	419 ± 35	2.97 ± 0.09	161 ± 3
	Tau2.54	12.0 ± 1.0	11.3 ± 0.7	12.3 ± 0.3d	328 ± 66	2.87 ± 0.07	150 ± 3
	Tau2.87	14.7 ± 0.9	10.7 ± 0.3	13.0 ± 0.6d	315 ± 43	3.20 ± 0.06	171 ± 7
29	Tau0.13	35.0 ± 0.0	29.5 ± 0.0	197 ± 0a	500 ± 0	3.10 ± 0.00	169 ± 0
	Tau0.56	88.3 ± 0.9	50.8 ± 14.9	90.8 ± 12.6b	289 ± 28	3.40 ± 0.15	162 ± 9
	Tau0.94	98.2 ± 22.7	48.3 ± 11.1	64.3 ± 16.5bc	286 ± 59	3.00 ± 0.55	143 ± 37
	Tau1.50	281 ± 123	68.7 ± 27.4	44.0 ± 18.6bcd	382 ± 30	3.57 ± 0.12	155 ± 12
	Tau2.02	109 ± 16	42.0 ± 4.3	55.3 ± 16.7bcd	336 ± 8	3.20 ± 0.15	161 ± 8
	Tau2.54	63.2 ± 4.6	38.7 ± 2.3	45.8 ± 11.8bcd	357 ± 29	3.27 ± 0.18	158 ± 7
	Tau2.87	107 ± 41	49.5 ± 14.4	47.5 ± 2.8bcd	337 ± 87	3.30 ± 0.17	146 ± 21
Main effect of temperature stress
19.5		15.1 ± 1.0b	12.5 ± 1.3b	13.4 ± 1.5b	360 ± 38	3.1 ± 0.1	165 ± 18
29		112 ± 30a	46.8 ± 5.4a	65.3 ± 9.4a	366 ± 27	3.3 ± 0.1	156 ± 2
Main effect of diet
	Tau0.13	22.5 ± 5.01	21.9 ± 7.58	106 ± 91a	490 ± 10	3.15 ± 0.03	167 ± 1
	Tau0.56	51.2 ± 16.6	31.8 ± 19.1	52.1 ± 38.8b	298 ± 9	3.35 ± 0.05	168 ± 6
	Tau0.94	55.9 ± 21.5	30.0 ± 18.3	39.0 ± 25.3b	316 ± 30	3.05 ± 0.05	149 ± 7
	Tau1.50	150 ± 80	41.5 ± 27.2	32.8 ± 12.2b	354 ± 28	3.43 ± 0.13	165 ± 10
	Tau2.02	61.3 ± 22.6	27.3 ± 14.7	34.2 ± 12.0b	377 ± 41	3.08 ± 0.12	161 ± 0.33
	Tau2.54	37.6 ± 11.6	25.0 ± 13.7	29.1 ± 9.2b	343 ± 14	3.07 ± 0.20	154 ± 4
	Tau2.87	60.9 ± 27.6	30.1 ± 19.4	30.3 ± 7.8b	326 ± 11	3.25 ± 1.62	159 ± 13
Two-way ANOVA (p-values)
Temperature		< 0.00011)	< 0.00011)	< 0.00011)	0.8554	0.2622	0.2877
Diet		0.0735	0.06909	< 0.00011)	0.0525	0.3943	0.8123
Temperature × Diet		0.0991	0.7606	< 0.00011)	0.7187	0.9035	0.8844

Values are significantly different (p < 0.05).
Values are means ± SEM from triplicate groups (n = 3) where the values in each column with different superscripts are significantly different (p < 0.05).
GOT, glutamic oxaloacetic transaminase; GPT, glutamic pyruvate transaminase; GLU, glucose; TG, triglycerides; TP total protein; TCHO, total cholesterol.