Table 2. List of primers used for the gene expression analysis for juvenile olive flounder after the acute temperature exposure test

Genes	Primer sequences	Tm (°C)	Accession number	Product size (bp)	Efficiency (%)	R²	Slope	Reference
β-actin	F: GGAATCCACGAGACCACCTACA	62.1	XM_020109620.1	264	99.9	0.9515	3.3250	Nie et al. (2021)
β-actin	R: CTGCTTGCTGATCCACATCTGC	62.1	XM_020109620.1	264	99.9	0.9515	3.3250	Nie et al. (2021)
hsp60	F: TGACTTCGGGAAAGTCGGTG	59.3	XM_020105844.1	2927	100.1	0.8904	3.3200	NCBI (2023)
hsp60	R: ACGATCTCCAGTGCACGTTT	57.3	XM_020105844.1	2927	100.1	0.8904	3.3200	NCBI (2023)
hsp70	F: TTCAATGATTCTCAGAGGCAAGC	58.9	XM_020089177.1	113	99.4	0.9994	3.3364	Mori et al. (2022)
hsp70	R: TTATCTAAGCCGTAGGCAATCGC	60.6	XM_020089177.1	113	99.4	0.9994	3.3364	Mori et al. (2022)
hsp90	F: GAGCGAGACAAGGAGGTGAG	61.4	XM_020091873.1	100	96.8	0.9944	3.4016	Lee et al. (2023)
hsp90	R: CTGGCTTGTCTTCGTCCTTC	59.3	XM_020091873.1	100	96.8	0.9944	3.4016	Lee et al. (2023)
wap65	F: AACCAAGGCTGTGGGAAGAAAGAG	63	XM_020105098.1	1727	98.6	0.9398	3.3550	Choi et al. (2010)
wap65	R: GTGTCCGTGGAAGCAGTAGTAGTG	64.4	XM_020105098.1	1727	98.6	0.9398	3.3550	Choi et al. (2010)

hsp60, Heat shock protein 60 kDa; hsp70, Heat shock protein 70 kDa; hsp90, Heat shock protein 90 kDa; wap65, Warm temperature acclimation-related protein 65 kDa.