Table 2. Statistical results of the general linear models testing for the effects of extreme temperature, vitamin C and their interaction on the response variables of cobia Rachycentron canadum

Effects	Specific growth rate			Food conversion ratio			Protein efficiency ratio			Protein productive value
	df1, df2	F-value	p-value	df1, df2	F-value	p-value	df1, df2	F-value	p-value	df1, df2	F-value	p-value
Temperature (Temp.)	1, 12	9.58	0.0091)	1, 12	9.70	0.0091)	1, 12	8.97	0.0111)	1, 12	6.44	0.0261)
Vitamin C	2, 12	9.59	0.0031)	2, 12	9.08	0.0041)	2, 12	9.63	0.0031)	2, 12	6.86	0.0101)
Temp. × vitamin C	2, 12	0.49	0.625	2, 12	0.23	0.798	2, 12	0.78	0.482	2, 12	0.123	0.885

Effects	Liver weight			Hepatosomatic index			Visera weight			Viscerosomatic index
	df1, df2	F-value	p-value	df1, df2	F-value	p-value	df1, df2	F-value	p-value	df1, df2	F-value	p-value
Temperature (Temp.)	1, 12	3.06	0.11	1, 12	0.71	0.415	1, 12	13.32	0.0031)	1, 12	2.83	0.119
Vitamin C	2, 12	0.90	0.432	2, 12	2.29	0.144	2, 12	10.63	0.0021)	2, 12	2.61	0.115
Temp. × vitamin C	2, 12	2.49	0.125	2, 12	4.19	0.0421)	2, 12	1.25	0.321	2, 12	7.4	0.0081)

Effects	Body protein			Lipid			Ash			Moisture
	df1, df2	F-value	p-value	df1, df2	F-value	p-value	df1, df2	F-value	p-value	df1, df2	F-value	p-value
Temperature (Temp.)	1, 12	6.44	0.0261)	1, 12	11.46	0.0051)	1, 12	32.85	< 0.0011)	1, 12	15.07	0.0021)
Vitamin C	2, 12	6.86	0.0101)	2, 12	0.23	0.797	2, 12	1.106	0.363	2, 12	2.32	0.141
Temp. × vitamin C	2, 12	0.12	0.885	2, 12	0.33	0.726	2, 12	2.12	0.163	2, 12	1.12	0.359

Effects	White blood cells			Red blood cells			Hemoglobin			Haematocrit
	df1, df2	F-value	p-value	df1, df2	F-value	p-value	df1, df2	F-value	p-value	df1, df2	F-value	p-value
Temperature (Temp.)	1, 12	26.43	< 0.0011)	1, 12	38.24	< 0.0011)	1, 12	3.64	0.081)	1, 12	0.14	0.716
Vitamin C	2, 12	37.33	< 0.0011)	2, 12	6.16	0.0141)	2, 12	33.88	< 0.0011)	2, 12	3.68	0.057
Temp. × vitamin C	2, 12	2.03	0.174	2, 12	3.79	0.0531)	2, 12	11.13	0.0021)	2, 12	4.35	0.0381)

Effects	Vitamin C in liver			Vitamin C in the body			Survival rate			Lysozyme activity
	df1, df2	F-value	p-value	df1, df2	F-value	p-value	df1, df2	F-value	p-value	df1, df2	F-value	p-value
Temperature (Temp.)	1, 12	6.63	0.0241)	1, 12	11.16	0.0061)	1, 12	15.12	0.0021)	1, 12	5.47	0.0371)
Vitamin C	2, 12	50.40	< 0.0011)	2, 12	139.82	< 0.0011)	2, 12	4.51	0.0351)	2, 12	6.53	0.0121)
Temp. × vitamin C	2, 12	5.94	0.0161)	2, 12	5.08	0.0251)	2, 12	4.51	0.0351)	2, 12	0.73	0.501

Significances (p < 0.05) are indicated.