Table 2. Primers used for quantitative real-time PCR
| Gene | Sequence (5′-3′) | Accession number |
| Trypsin-F | TCCAAGATCATCCAACACGA | Y15040 |
| Trypsin-R | GACCCTGAGCGGGAATATC |
| Chymotrypsin-F | GGCTCTCTTCATCGACG | X66415 |
| Chymotrypsin-R | CGTGAGTGAAGAAGTCGG |
| Amylase-F | CAAACTGGTTGGGCTGAACG | KM077135.1 |
| Amylase-R | TAGTACGACGACATCACGCG |
| IGF-I-F | GGCTTCAGCGTCAGGTGTTCCC | HAAW01015949 |
| IGF-I-R | ACCCTTCCCGCAGATGTAGCAG |
| IGF-BP-F | GTGGGCAGGGACCAAATC | KP420228 |
| IGF-BP-R | TCAGTTACCACCAGCGATT |
| proPO-F | CGGTGACAAAGTTCCTCTTC | AY723296 |
| proPO-R | GCAGGTCGCCGTAGTAAG |
| Pen-3a-F | CACCCTTCGTGAGACCTTTG | Y14926 |
| Pen-3a-R | AATATCCCTTTCCCACGTGAC |
| β-actin-F | CCACGAGACCACCTACAAC | AF300705.2 |
| β-actin-R | AGCGAGGGCACTGATTTC |
IGF-I, insulin-like growth factor-I; IGF-BP, insulin-like growth factor-binding protein; proPO, prophenoloxidase; Pen-3a, penaeidin-3a.