Table 2. Amino acid identities of putative protein between Pangasianodon hypophthalmus and Pangasius nasutus with other vertebrates and GenBank accession numbers of GnRH1 and GnRH2 used in this study

Class order GnRH1 Identities (%) GenBank accession number GnRH2 Identities (%) GenBank accession number
P. hypophthalmus P. nasutus P. hypophthalmus P. nasutus
Osteichthyes
 Siluriform
  Pangasius nasutus (patin buah) 99.9 - OP104320 99.9 - OP104321
  Pangasianodon hypophthalmus (striped catfish) - 99.9 OQ685894 - 99.9 OQ685895
  Hemibagrus nemurus (river catfish) 99.7 99.7 OP115879.1 99.7 99.7 OP115878.1
 Pedicted Pangasianodon hypophthalmus (striped catfish) 100 99.9 XM_026924976.1 99.9 99.9 XM_034311363.1
  Ictalurus punctatus (channel catfish) 100 99.9 XM_047155459.1 99.8 99.8 XM_017493147.2
  Silurus meridionalis (southern catfish) 99.7 99.6 XM_046871947.1 99.8 99.8 XM_046868054.1
 Perciformes
  Tricoated trichopterus (three spot gourami) 98.1 98 KF113107.1 99.2 99.3 KF113108.1
  Larimichthys crocea (yellow crocker) 97.9 97.8 NM_001303356.1 99.2 99.3 XM_010743322.3
  Seriola lalandi dorsalis (great amberjack) 98 97.9 XM_023394165.1 99.2 99.3 XM_023428006.1
  Lates calcarifer (barramudi) 98.2 98 XM_018668358.1 99.2 99.3 XM_018700270.1
 Cyprinodontiformes
  Nothobranchius furzeri (Turqoise killerfish) 98.0 97.9 XM_015972478.1 99.2 99.2 XM_015959339.1
  Xiphophorus maculatus (southern platyfish) 98.2 98.2 XM_005811760.3 99.1 99.2 XM_005808463.2
  Poecilia reticulata (guppy) 98.1 98 XM_008417516.2 99.1 99.2 XM_008409509.2
Clupeiformes
  Engraulis japonicus (Japanese anchovy) 97.7 97.6 JX406273.1 99.1 99.1 JX406274.1
  Sardinops melanostictus (Japanese pilchard) 98.3 98.2 MW252048.1 99.3 99.3 MW252049.1
Anguilliformes
  Anguilla anguilla (European eel) 98.3 98.2 GU332301.1 99.1 99.2 GU332293.1
Amphibia
 Anura
  Xenopus tropicalis (western clawed frog) 97.5 97.5 EF166084.1 98.3 98.4 NM_001114078.1
Mammalia
 Rodentia
  Urocitellus parryii (arctic ground squirrel) 97.4 97.4 XM_026411610.1 97.3 97.3 XM_026409697.1
  Octodon degus (Degu) 97.7 97.8 XM_004630672.1 97.2 97.3 XM_004634317.1
Primates
  Macaca mulatta (rhesus monkey) 97.8 97.7 NM_001195436.2 97.7 97.8 NM_001034202.1
  Theropithecus gelada (gelada monkey) 97.8 97.7 XM_025394633.1 97.5 97.6 XM_025400095.1
  Homo sapiens (human) 97.8 97.7 NM_001083111.2 97.6 97.7 NM_001501.2
GnRH, gonadotropin-releasing hormone.