Table 4. Substrate-specific activity of LhGSTO1 and LhGSTK1 at 25℃ toward different substrates

Substrate	pH	λ_max (nm)	Molecular extinction coefficient (ε) (mM/cm)	Specific activity (μmol/min//mg)
Substrate	pH	λ_max (nm)	Molecular extinction coefficient (ε) (mM/cm)	rLhGSTO1	rLhGSTK1
CDNB	6.5	340	9.6	2.65 ± 0.46	13.52 ± 2.39
DCNB	7.5	345	8.5	ND	ND
4-NPB	6.5	310	1.2	ND	ND
4-NBC	6.5	310	1.9	ND	ND

The four substrates were used to monitor GSTs activity.
CDNB, 1-chloro-2,4-dinitrobenzene; DCNB, 1,2-dichloro-4-nitrobenzene; 4-NPB, 4-nitrophenethyl bromide; 4-NBC, 4-nitrobenzyl chloride; ND, not detected; GSTs, glutathione S-transferases.