Table 2. Fertilizing capacity of cryopreserved Chirostoma jordani sperm
Cryopreserved semen |
Group | Batch | Volume (µL) | Sperm concentration per straw (cells per/µL) | Sperm motility per straw | Activating solution (µL) | Oocytes |
DMSO | 1 | 10.0 | 18,000,000 | 9,054,000 | 50.0 | 60 |
2 | 5.0 | 9,000,000 | 4,527,000 | 25.0 | 60 |
3 | 2.5 | 4,500,000 | 2,263,500 | 12.5 | 60 |
4 | 1.25 | 2,250,000 | 1,131,750 | 6.25 | 60 |
MeOH | 1 | 10.0 | 18,000,000 | 9,594,000 | 50.0 | 60 |
2 | 5.0 | 9,000,000 | 4,797,000 | 25.0 | 60 |
3 | 2.5 | 4,500,000 | 2,398,500 | 12.5 | 60 |
4 | 1.25 | 2,250,000 | 1,199,250 | 6.25 | 60 |
EG | 1 | 10.0 | 18,000,000 | 9,630,000 | 50.0 | 60 |
2 | 5.0 | 9,000,000 | 4,815,000 | 25.0 | 60 |
3 | 2.5 | 4,500,000 | 2,407,500 | 12.5 | 60 |
4 | 1.25 | 2,250,000 | 1,203,750 | 6.25 | 60 |
DMSO, dimethyl sulfoxide; MeOH, methanol; EG, ethylene glycol.