| Fresh semen | ||||
|---|---|---|---|---|
| Group | Semen (µL) | Sperm concentration (cells per/µL) | Activating solution (µL) | Oocytes |
| 1 | 0.4 | 720,000 | 2.0 | 60 |
| 2 | 0.2 | 360,000 | 1.0 | 60 |
| 3 | 0.1 | 180,000 | 0.5 | 60 |
| 4 | 0.08 | 144,000 | 0.4 | 60 |
| 5 | 0.06 | 108,000 | 0.3 | 60 |
| 6 | 0.04 | 72,000 | 0.2 | 60 |
| 7 | 0.02 | 36,000 | 0.1 | 60 |
| 8 | 0.01 | 18,000 | 0.05 | 60 |