Table 1. Formulation and proximate composition (g/kg, dry matter) of the experimental diets for juvenile parrot fish (Oplegnathus fasciatus)

Ingredients	Diets
Ingredients	L80	L120	L160	L200	L240
Fish meal¹⁾	450	450	450	450	450
Casein²⁾	85	90	95	100	105
Soybean meal¹⁾	110	110	110	110	110
Wheat flour	220	190	160	130	90
Yeast	20	20	20	20	20
Mineral premix³⁾	10	10	10	10	10
Vitamin premix⁴⁾	10	10	10	10	10
Squid liver oil⁵⁾	35	75	115	155	195
Carboxymethyl cellulose⁶⁾	10	10	10	10	10
Cellulose	50	35	20	5	0
Proximate composition
Dry matter	908	931	900	928	947
Crud protein	513	520	518	518	527
Crud lipid	79	119	160	193	228
Ash	91	90	94	97	93
Nitrogen-free extract⁷⁾	305	268	231	194	160
Gross energy (MJ/kg)⁸⁾	15.2	16.4	17.5	18.7	19.7
P/E ratio (mg protein/kcal)	138	128	120	113	107

Suhyup Feed, Uiryeong, Korea.
United States Biochemical (USB), Cleveland, OH, USA.
Mineral premix (g/kg): magnesium sulfate heptahydrate, 80; sodium phosphate monobasic dihydrate, 370; potassium chloride, 130; Ferric citrate, 40; zinc sulfate heptahydrate, 20; Ca-lactate, 356; copper chloride, 0.2; aluminium chloride hexahydrate, 0.15; sodium thiosulfate pentahydrate, 0.01; manganese sulfate monohydrate, 2; cobalt chloride hexahydrate, 1.
Vitamin premix (g/kg of mixture): L-ascorbic acid monophosphate, 100; α tocopheryl acetate, 20; thiamin hydrochloride, 4; riboflavin, 4.4; pyridoxine hydrochloride, 4; niacin, 30; pantothenic acid hemicalcium salt, 14.5; myo-inositol, 40; biotin, 0.2; folic acid, 0.48; menadione, 0.2; retinyl acetate, 1; cholecalficerol, 0.05; cyanocobalamin, 0.01.
E-Wha oil, Busan, Korea.
Sigma-Aldrich, St. Louis, MO, USA.
Nitrogen free extract was calculated through the following formula: NFE = 1,000 – (moisture + crude protein + crude lipid + ash).
The gross energy value of each diet was calculated on the basis of physiological fuel value, i.e., 16.7 KJ/g proteins or carbohydrates and 37.7 KJ/g lipids.