Table 4. Non-specific immune parameters of red seabream, Pagrus major, fed experimental diets for 12 weeks

	Lysozyme¹⁾	SOD²⁾	NBT³⁾	MPO⁴⁾	Anti-protease⁵⁾
AA30	12.2^b	57.1^b	1.08^c	1.81	25.1
AA90	15.2^b	63.5^ab	1.14^ab	1.85	27.0
AA300	26.9^a	69.9^a	1.15^a	1.85	29.8
CP30	13.8^b	66.9^a	1.13^abc	2.04	30.6
CP90	15.3^b	70.0^a	1.15^a	2.00	31.3
CP300	24.5^ab	70.7^a	1.09^bc	1.86	31.4
SEM⁶⁾	8.29	6.86	0.04	0.32	6.41
Analysis of variance
Ascorbic acid level	0.015	0.038	0.101	0.821	0.916
Ascorbic acid source	0.946	0.029	0.806	0.254	0.500
Level × source	0.864	0.298	0.013	0.707	0.957

AA30, AA90, and AA300 were 30, 90, and 300 mg/kg of ascorbic acid incorporated diet groups and CBP30, CBP90, and CBP300 were citrus by-products incorporated diets replacing ascorbic acid levels in the AA diets.
Values are means from triplicate groups of fish where the values in each column with the different superscripts are significantly different (p < 0.05).
Lysozyme (μg/mL).
Superoxide dismutase enzyme activity (% Inhibition).
Nitroblue tetrazolium activity (absorbance at 540 nm).
Myeloperoxidase activity (absorbance at 450 nm).
Antiprotease activity (% Inhibition).
Pooled standard error of the mean.
AA, ascorbic acid; CBP, citrus by-product.