Table 1. Proximate composition of the experimental diets (%, dry matter basis) for red seabream (Pagrus major)

	CON	MX20	MX40
Fish meal1)	60.00	40.0	20.0
Meat meal		5.00	10.0
Chicken byproduct meal		5.00	10.0
Soy protein concentrate		5.00	10.0
Corn gluten	5.00	5.00	10.0
Wheat flour	19.00	24.61	22.52
Squid liver powder	6.00	6.00	6.00
Fish oil		2.30	4.60
Soybean oil	6.00	2.25	1.20
Vitamin premix2)	1.50	1.50	1.50
Mineral premix3)	2.00	2.00	2.00
Choline	0.50	0.50	0.50
Met		0.40	0.80
Lys		0.44	0.88
Proximate composition
Dry matter	95.1	94.2	94.4
Protein	49.9	49.4	47.4
Lipid	13.2	10.9	11.7
Ash	11.6	10.3	9.35

Anchovy fish meal (crude protein, 65.9; crude lipid 9.41).
Vitamin mixture composition (unit/kg mix): ascorbic acid, 6,400 mg; tocopherol acetate, 37,500 mg; thiamin nitrate, 5,000 mg; riboflavin, 10,000 mg; pyridoxine hydrochloride, 5,000 mg; nicotinic acid, 37,500 mg; Ca-D-pantothenate, 17,500 mg; inositol, 75,000 mg; biotin, 50 mg; folic acid, 2,500 mg; menadione sodium bisulfite, 2,500 mg; retinol acetate, 5,000,000 IU; cholecalciferol, 1,000,000 IU; cyanocobalamin, 25 mg; riboflavin, 10,000 mg.
Mineral mixture composition (g/kg mix); ferrous fumarate, 12.5; manganese sulfate, 11.3, ferrous sulfate, 20; cupric sulfate, 1.25; cobaltous sulfate, 0.75; zinc sulfate, 13.75; calcium iodate, 0.75; magnesium sulfate, 80.2; aluminum hydroxide, 0.75.
CON, considered as control.