Table 1. Detection of KHV, CEV, and CyHV-2 from Cyprinids between 2021 and 2022
| Source | Species | Group code | Date | Sample number | Body length (cm) | Body weight (g) | KHV | CEV | CyHV-2 | Virus isolate2) |
| Thymidine kinase1) | SphI-51) | Partial 4a1) | Helicase1) |
| Domestic | Common carp | GrCc-2204 | APR-2022 | 30 | 5.88 ± 0.45 | 0.89 ± 0.36 | ND | ND | ND | ND | ND |
| Koi carp | GrKc-2104 | APR-2021 | 10 | 5.54 ± 0.54 | 1.36 ±0.34 | ND | ND | ND | ND | ND |
| JcKc-2104 | APR-2021 | 10 | 15.05 ± 1.00 | 45.33 ± 9.16 | ND | ND | ND | ND | ND |
| ScKc-2105 | MAY-2021 | 30 | 9.90 ± 1.43 | 15.71 ± 6.46 | 29/30 (96.7%) | 15/30 (50.0%) | 2/30 (6.7%) | ND | ScKc-2105-K ScKc-2105-CE |
| BcKc-2106 | JUN-2021 | 30 | 4.92 ± 0.12 | 0.85 ± 0.26 | ND | ND | ND | ND | ND |
| BsKc-2109 | SEP-2021 | 30 | No data | No data | ND | ND | ND | ND | ND |
| ScKc-2204 | APR-2022 | 30 | 13.97 ± 0.10 | 32.44 ± 8.68 | ND | ND | ND | ND | ND |
| JcKc-2205 | MAY-2022 | 30 | 13.8 ± 1.46 | 33.00 ± 8.87 | ND | ND | ND | ND | ND |
| GhKc-2207 | JUL-2022 | 30 | 17.10 ± 1.94 | 46.88 ± 17.70 | ND | 4/30 (13.3%) | 25/30 (83.3%) | ND | GhKc-2207-K GhKc-2207-CE |
| Goldfish | BsGf-2106 | JUN-2021 | 20 | 10.83 ± 0.51 | 11.46 ± 1.81 | ND | ND | ND | ND | ND |
| JcGf-2104 | MAY-2021 | 10 | No data | No data | ND | ND | ND | ND | ND |
| GrGf-2104 | MAY-2021 | 10 | No data | No data | ND | ND | ND | ND | ND |
| Imported (Thailand) | Koi carp | ThGf-2101C | JAN-2021 | 8 | 9.16 ± 0.50 | 8.69 ± 1.07 | ND | ND | ND | ND | ND |
| ThGf-2101D | JAN-2021 | 8 | 6.66 ± 0.45 | 3.15 ± 0.65 | ND | ND | ND | ND | ND |
| ThGf-2205E | MAY-2022 | 30 | 8.05 ± 0.70 | 6.55 ± 1.48 | ND | ND | ND | ND | ND |
| ThGf-2206F | JUN-2022 | 30 | 8.89 ± 0.56 | 6.59 ± 1.05 | ND | ND | ND | ND | ND |
| Goldfish | ThGf-2101A | JAN-2021 | 8 | 4.08 ± 0.18 | 2.28 ± 0.37 | ND | ND | ND | 2/8 (25.0%) | ThGf-2101A-Cy |
| ThGf-2101B | JAN-2021 | 8 | 7.53 ± 0.37 | 2.73 ± 0.23 | ND | ND | ND | 3/8 (37.5%) | ThGf-2101B-Cy |
| ThGf-2107 | JUL-2021 | 13 | 5.63 ± 0.29 | 5.97 ± 0.90 | ND | ND | ND | 12/13 (92.3%) | ThGf-2107-Cy |
Target gene for PCR amplification.
The virus isolates identified through PCR were denoted by the final one or two letters (K, CE, and Cy), which represent KHV, CEV, and CyHV-2, respectively.
KHV, koi herpesvirus; CEV, carp edema virus; CyHV-2, cyprinid herpesvirus-2; PCR, polymerase chain reaction; ND, not detected.