| 1 | HPLC | Mass spectrometry FAB/MS with VG ZAB-2HF | Litoria genimaculata | Australia | Naculatin and maculatin 1.1 | (Rozek et al., 1998) |
| 2 | HPLC | Electron ray ionization quadrupole ion trap mass spectrometry (ESIMS) | Bombina variegata | Germany | Bomesin | (Marenah et al., 2004b) |
| 3 | HPLC reverse phase (RP-HPLC) | Mass spectrometry | Hylarana guntheri | Asia | Brevinin | (Conlon et al., 2008a) |
| 4 | RP-HPLC | Mass spectrometry | Xenopus laevis | Nigieria | Magainin | (Zasloff, 1987a) |
| 5 | RP-HPLC | Mass spectrometry | Rana tsushimensis | Japan | Stejneger | (Conlon et al., 2006) |
| 6 | RP-HPLC | Mass spectrometry | Rana areolata | American | Esculentin-1a | (Di Grazia et al., 2015) |
| 7 | RP-HPLC | Mass spectrometry | Leptodactylus syphax | Brazil | Syphaxin (Spx) | (Dourado et al., 2007) |
| 8 | Sephadex G-10 and G-50 column chromatography, RP-HPLC | Mass spectrometry | Bombina orientalis | Korea | Bombesin (Bbs-LS) | (Park et al., 1989) |
| 9 | Sephadex G-50 column, HPLC | Mass spectrometry | Rana nigromaculata | Korea | Nigrocin | (Park et al., 2001) |
| 10 | Thin layer chromatography, gas chromatography | Mass spectrometry | Dendrobatid | South America | Alkaloid | (Daly et al., 1978; Daly et al., 2002) |