Table 3. Species specific primers for multiplex PCR informations

Sets	Name	Sequence (5’→3’)	Target species	Tm (°C)	Product size (bp)
Set1	Gs-F344	GGTGAACCGTTTACCCTCCC	Glyptocephalus stelleri	58	1,064
	Tk-F475	ATCAACATAAAACCGACAACC	Tanakius kitaharae		993
	Ca-F700	ATACATTCTTATTCTCCCAGGT	Clidoderma asperrimum		709
	Eg-F835	CCACCACATGTTCACGGTA	Eopsetta grigorjewi		571
	Cp-F1147	CGTACACTGATTCCCCCTT	Cleisthenes pinetorum		259
Set2	Ph-F403	CCTCACCATTTTCTCTCTTCAT	Pleuronectes herzensteini	62	1,006
	Ma-F793	GGCTATGATGGCTATCGGC	Microstomus achne		613
	Ps-F1096	CACTTCCACTATGTCCTATCA	Platichthys stellatus		312
Set3	Kb-F594	TGCTACTAACAGACCGCAACT	Kareius bicoloratus	62	815
	Py-F892	TGCCACAATAATTATTGCCATT	Pleuronectes yokohamae		517
	La-F1249	CCCAACATTTTCTTGGCCTT	Limanda aspera		159
	GJM_128-F	GCACACGCCTTTGTAATAAT	Common primers	54	1,242
	GJM_1370-R	CGTTTGGCTGTAAATGCTTC

PCR, polymerase chain reaction; Tm, temperature.