Table 2. Information of COI region common primers from mtDNA sequences of the eleven species of family Pleuronectidae

Oligo name Sequence (5’ → 3’) Mer Tm (°C) GC (%) Product size
GJM-F128 GCA CAC GCC TTT GTA ATA AT 20 59 40 1,242 bp
GJM-R1370 CGT TTG GCT GTA AAT GCT TC 20 62 45
COI, cytochrome c oxidase subunit I; mtDNA, mitochondrial DNA; Tm, temperature.