Table 5. Alpha diversity index

Storage treatment	Observed ASVs	Shannon index	Simpsons index
A2	325	2.35	0.83
A10	319	3.08	0.81
C1	104	3.18	0.16

Alpha diversity is calculated using diversity metrics in QIIME 2. The eastern little tuna sample A2, storage treatment at 2°C; A4, storage treatment at 4°C; A10, storage treatment at 10°C; C1, fish temperature 30°C.
ASV, amplicon sequence variant; QIIME, Quantitative Insights into Microbial Ecology.