Table 3. Effect of PBPs and nanoencapsulated PBPs on the chemical and microbial changes of fish burgers during storage (4°C)

Variable	Time	Treatment
Variable	Time	Control (0%)	Incorporated with 2.5% PBPs	Incorporated with 5% PBPs	Incorporated with 2.5% encapsulated PBPs	Incorporated with 5% encapsulated PBPs
TVB-N	0	3.03 ± 0.07^Ag	3.05 ± 0.04^Ag	3.04 ± 0.04^Ag	3.03 ± 0.7^Ag	2.92 ± 0.02^Ag
	3	4.43 ± 0.04^Af	4.29 ± 0.05^Bf	4.18 ± 0.08^Cf	4.02 ± 0.08^Df	3.92 ± 0.02^Df
	6	5.83 ± 0.01^Ae	5.71 ± 0.01^Ae	5.05 ± 0.21^Be	4.48 ± 0.04^Ce	4.40 ± 0.02^Ce
	9	10.48 ± 0.23^Ad	8.48 ± 0.07^Bd	7.72 ± 0.09^Cd	5.99 ± 0.05^Dd	5.23 ± 0.03^Ed
	12	16.36 ± 0.12^Ac	9.26 ± 0.06^Bc	8.03 ± 0.09^Cc	6.98 ± 0.10^Dc	6.38 ± 0.05^Ec
	15	20.66 ± 0.20^Ab	11.57 ± 0.26^Bb	9.62 ± 0.20^Cb	8.17 ± 0.07^Db	7.50 ± 0.03^Eb
	18	30.34 ± 1.09^Aa	12.76 ± 0.22^Ba	10.48 ± 0.23^Ca	9.54 ± 0.53^Ca	8.34 ± 0.09^Da
pH	0	6.77 ± 0.03^ABe	6.74 ± 0.04^Cf	6.67 ± 0.03^Df	6.79 ± 0.02^ABe	6.80 ± 0.02^Ae
	3	6.83 ± 0.03^Bf	6.89 ± 0.01^Ae	6.79 ± 0.03^Be	6.88 ± 0.02^Abd	6.89 ± 0.02^Ad
	6	6.73 ± 0.03^Ae	6.77 ± 0.04^Af	6.76 ± 0.02^Ae	6.78 ± 0.01^Ae	6.78 ± 0.02^Ae
	9	7.12 ± 0.02^Ad	7.11 ± 0.01^Bd	6.92 ± 0.02^Cd	6.82 ± 0.02^De	6.79 ± 0.03^Ee
	12	7.42 ± 0.01^Ac	7.25 ± 0.01^Bc	7.25 ± 0.03^Bc	7.02 ± 0.07^Cc	6.97 ± 0.03^Cc
	15	7.78 ± 0.02^Ab	7.72 ± 0.02^Ba	7.72 ± 0.02^Bb	7.17 ± 0.01^Cb	7.12 ± 0.01^Db
	18	7.97 ± 0.03^Aa	7.64 ± 0.03^Bb	7.62 ± 0.01^Ba	7.22 ± 0.02^Ca	7.18 ± 0.01^Da
TVC	0	3.07 ± 0.24^Ag	2.79 ± 0.07^Bg	2.55 ± 0.09^BCg	2.36 ± 0.14^Cf	2.07 ± 0.12^De
	3	4.24 ± 0.02^Af	3.65 ± 0.06^Bf	3.22 ± 0.02^Cf	3.11 ± 0.02^De	2.75 ± 0.05^Ed
	6	5.86 ± 0.03^Ae	4.96 ± 0.05^Be	3.91 ± 0.18^Ce	3.66 ± 0.14^Dd	2.97 ± 0.06^Ecd
	9	6.77 ± 0.03^Ad	5.95 ± 0.22^Bd	4.33 ± 0.03^Cd	4.02 ± 0.09^Dc	3.21 ± 0.01^Ed
	12	7.78 ± 0.01^Ac	6.60 ± 0.17^Bc	5.50 ± 0.35^Cc	5.18 ± 0.03^Cb	4.14 ± 0.05^Dc
	15	8 ± 0.11^Ab	7.33 ± 0.03^Bb	6.28 ± 0.20^Cb	5.85 ± 0.08^Da	5 ± 0.26^Eb
	18	8.37 ± 0.05^Aa	7.73 ± 0.29^Ba	6.80 ± 0.09^Ca	5.97 ± 0.5^Da	5.42 ± 0.25^Ea
PTC	0	2.72 ± 0.10^Af	2.55 ± 0.05^Bf	2.45 ± 0.05^Bg	2.43 ± 0.06^Bg	2.23 ± 0.06^Cf
	3	3.80 ± 0.05^Ae	3.43 ± 0.06^Be	3.17 ± 0.05^Cf	2.95 ± 0.05^Df	2.80 ± 0.05^Ee
	6	4.87 ± 0.03^Ad	3.97 ± 0.06^Bd	3.45 ± 0.05^Ce	3.32 ± 0.03^De	3.02 ± 0.13 ^Ee
	9	5.62 ± 0.34^Ac	4.85 ± 0.05^Bc	4.10 ± 0.26^Cd	4 ± 0.20^Cd	3.33 ± 0.29^Dd
	12	6.93 ± 0.13^Ab	6.57 ± 0.51^Ab	5.47 ± 0.12^Bc	4.93 ± 0.15^Cc	4.17 ± 0.15^Dc
	15	7.67 ± 0.15^Aa	7.15 ± 0.13^Ba	5.93 ± 0.15^Cb	5.28 ± 0.08^Db	4.78 ± 0.08^Eb
	18	7.92 ± 0.08 ^Aa	7.33 ± 0.06^Ba	6.53 ± 0.21^Ca	5.88 ± 0.10^Da	5.75 ± 0.05^Da

Values are mean ± SD of three determinations.
Means bearing different superscripts in the same row are significantly different (p < 0.05).
Means bearing different superscripts in the same column are significantly different (p < 0.05).
PBPs, phycobiliproteins; TVB-N, total volatile basic nitrogen; TVC, total viable counts; PTC, psychrotrophic bacterial counts.