Table 2. Average copy numbers of recovered environmental DNA by the different pretreatment conditions

Conditions	Variables	AjaCOI	AjaβActin	Ratio COI/β-Actin
Incubation time	10 min	68,994.22 ± 14,764.21	30,741.12 ± 15,065.30	3.26 ± 2.08
	30 min	167,206.37 ± 36,622.03	55,266.81 ± 21,067.47	3.67 ± 0.88
	60 min	302,848.32 ± 198,425.66	60,293.16 ± 35,517.03	4.61 ± 0.46
Water temperature	18 ± 1°C	90,009.01 ± 51,119.88	16,830.75 ± 7,291.28	4.96 ± 0.89
	21 ± 1°C	183,524.44 ± 59,967.08	26,828.46 ± 6,845.50	6.43 ± 0.80
	24 ± 1°C	119,977.67 ± 29,757.28	39,610.02 ± 9,723.73	3.02 ± 0.27
	27 ± 1°C	231,244.25 ± 40,613.00	63,745.12 ± 19,075.34	3.89 ± 0.49
	30 ± 1°C	62,754.76 ± 5,339.14	19,271.36 ± 3,477.84	3.31 ± 0.32
Volume of water	1-fold	5,543,172.26 ± 4,242,047.81	336,312.71 ± 172,710.84	13.59 ± 5.64
	5-fold	918,628.01 ± 282,980.04	112,278.35 ± 46,356.80	9.16 ± 2.89
	10-fold	242,669.63 ± 8,892.82	38,442.42 ± 11,520.72	7.42 ± 1.95
	20-fold	44,760.37 ± 13,919.24	6,527.86 ± 3,284.15	8.23 ± 1.87

Values are presented as mean ± SD.
COI, cytochrome c oxidase I.