Table 3. The chloramphenicol resistance gene detection in Escherichia coli isolated from aquaculture farm and rivers
Lane | E. coli isolate | Chloramphenicol resistant gene |
cat I (349 bp) | cat II (567 bp) | cat III (275 bp) | cat IV (451 bp) |
1 | Positive control | + | + | – | – |
2 | RBK-S2-A | – | + | – | + |
3 | RDM-S1-A | – | + | – | + |
4 | RDM-S2-A | + | + | – | – |
5 | RDM-S3-A | + | + | – | – |
6 | RWF-S1-A | – | + | – | + |
7 | RSN-S1-A | – | + | + | – |
8 | RSN-S2-A | – | + | + | – |
9 | FAS-RV1(S) | – | + | – | + |
10 | FBK2-OLT(B) | – | + | – | + |
11 | FBK2-K2 | – | + | + | – |
Positive control, DUS-WF-6; +, indicates the presence of cat genes; –, indicate the absence of cat genes.