Table 3. Proximate and amino acid compositions of the reference and test diets fed to olive flounder

	Reference diet	Test diets (70% reference + 30% ingredient)
		PM	JMM	AM	CM	SM	SEM	TM	MM	SLM
Proximate analysis (% of dry matter)
Crude protein	53.1	56.2	57.6	57.1	58.0	59.5	57.8	55.1	55.0	51.2
Crude lipid	10.4	8.1	9.3	9.7	10.0	10.2	10.3	9.3	11.7	12.0
Ash (% DM)	15.2	17.0	15.1	15.1	14.4	12.3	14.6	17.0	16.5	12.7
NFE1)	21.3	18.2	17.6	17.7	17.4	17.7	17.1	18.2	16.4	23.6
Gross energy (MJ/kg)2)	20.2	19.5	20.2	20.2	20.5	21.0	20.5	19.7	20.3	20.7
Amino acids (% of protein)
Arg	6.2	6.4	6.4	6.5	6.4	6.4	6.4	6.0	5.8	5.8
His	3.7	3.0	3.4	3.3	3.0	3.0	3.0	3.0	3.0	2.9
Ile	4.5	3.7	3.8	3.8	3.8	3.7	3.8	3.7	3.5	3.6
Leu	8.2	6.8	6.8	6.8	6.8	6.7	6.8	6.5	6.5	6.4
Lys	8.0	7.6	7.8	7.9	7.9	7.7	7.9	6.9	7.0	6.7
Met + Cys	4.8	4.1	3.9	4.0	4.1	4.0	4.0	3.9	3.7	3.8
Phe + Tyr	7.5	6.2	6.3	6.4	6.4	6.2	6.3	6.1	5.9	6.1
Thr	5.0	4.7	4.7	4.6	4.8	4.7	4.7	4.5	4.3	4.3
Val	5.6	5.4	5.4	5.5	5.6	5.3	5.5	5.2	5.0	4.9

NFE was calculated by difference.
Based on 23.4 MJ/kg protein, 39.2 MJ/kg lipid and 17.2 MJ/kg NFE.
PM, pollock meal; JMM, jack mackerel meal; AM, anchovy meal; CM, cod meal; SM, sardine meal; SEM, sand eel meal; TM, tuna meal; MM, meat meal; SLM, squid liver meal; NFE, nitrogen-free extract.