Table 5. Results by microscopic examination, PCR assays with Kudoa septempunctata in positive samples

Food type Region K. septempunctata spore K. septempunctata detected by PCR Identity of K. septempunctata
18S rDNA 28S rDNA AB731754.1 (%) AB731755.1 (%)
Sashimi-1 Wando 8.4 × 105 /g P P 99.74 100
Sashimi-2 Samcheonpo 8.0 × 104 /g ND ND
Sashimi-3 Samcheonpo 9.0 × 105 /g P P 99.48 99.72
Sashimi-4 Jeju 6.0 × 105 /g P P 98.45 99.45
Sashimi-5 Jeju 2.0 × 104 /g ND ND
Sashimi-6 Jeju 1.8 × 105 /g ND ND
Sashimi-7 Jeju 5.0 × 105 /g P P 98.43 100
Sashimi-8 Jeju 2.0 × 105 /g ND ND
Sashimi-9 Jeju 2.0 × 105 /g ND ND
Sashimi-10 Jeju 1.0 × 104 /g ND ND
Sashimi-11 Jeju 3.4 × 105 /g P P 99.74 99.72
PCR, polymerase chain reaction; P, positive; ND, not detected.