Table 1. Primers and probe used in this study

Genomic regions	Primers	Sequence (5'–3')	Amplicon (bp)	Purpose	Reference
Pst I fragment	1-F	CTC AAA CAC TCT GGC TCA TC	570	Detection¹⁾	Kurita et al. (1998)
Pst I fragment	1-R	GCA CCA ACA CAT CTC CTA TC	570
DNA polymerase gene	4-F	CGG GGG CAA TGA CGA CTA CA	568
DNA polymerase gene	4-R	CCG CCT GTG CCT TTT CTG GA	568
Major capsid protein	RSIV-MCP1F	ATG TCT GCR ATC TCA GGT GC	1,362	Cloning²⁾	Kim et al. (2018)
	RSIV-MCP1362R	TYA CAG GAT AGG GAA GCC TGC	1,362	Cloning²⁾	Kim et al. (2018)
	MqF	GGC GAC TAC CTC ATT AAT GT	141	Detection andquantification³⁾	Jin et al. (2014)
	MqR	CCA CCA GGT CGT TAA ATG A	141	Detection andquantification³⁾	Jin et al. (2014)
	RSIV 1094F	CCA GCA TGC CTG AGA TGG A	128	Detection and quantification⁴⁾	This study
	RSIV 1221R	GTC CGA CAC CTT ACA TGA CAG G
	RSIV 1177 probe	FAM-TAC GGC CGC CTG TCC AAC G-BHQ1

PCR assays for RSIVD in Manual of Diagnostic Tests for Aquatic Animal in OIE.
Construction of standard curve for quantitative analysis.
SYBR Green-based real-time PCR assay.
TaqMan probe-based real-time PCR assay.
RSIV, red sea bream virus; MCP, major capsid protein; RSIV, red sea bream virus; PCR, polymerase chain reaction.