Table 2
Effects of temperature and basal media on the culture of Sebastes schlegeli ovary-dissociated cells

Factors

Group

No. of cell populations tested

No. (%)a of cell populations initially attached

No. (%)a of cell populations subcultured to

Passage 1

Passage 2

Passage 3

Passage 4

Whole replicates

Merged

42

42 (100)

27 (64)

9 (21)

3 (7)

1 (2)

Basal media

L15

21

21 (100)

18 (86)

8 (38)

3 (14)

1 (5)

DMEM

21

21 (100)

9 (43)

1 (5)

0 (0)

0 (0)

P-value

-

0.003

0.0076

0.0754

0.3233

Temperature

15

14

14 (100)

10 (71)

4 (29)

1 (7)

0 (0)

(°C)

20

14

14 (100)

9 (64)

3 (21)

1 (7)

0 (0)

25

14

14 (100)

8 (57)

2 (14)

1 (7)

1 (7)

P-value

-

0.7475

0.6717

1.0000

0.3771