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E-FAS, vol. 18, no. 4, pp.341-347, December, 2015
DOI. http://dx.doi.org/10.5657/FAS.2015.0341

Investigation of Antimicrobial, Antioxidant and Hemolytic Activity of Water-soluble Extract of Mottled Anemone
Urticina crassicornis

Ye Jin Lee, Chan-Hee Kim, Hye Young Oh, Hye-Jin Go and Nam Gyu Park*
Department of Biotechnology, Pukyong National University, Busan 48513, Korea

Sea anemone (phylum Cnidarian) has been recognized as a new source of marine bioactive compounds due to the growth of marine bioprospecting. Studies of bioactive compounds in cnidarians have focused mainly on antitumor compounds, such as toxins in aqueous extracts and terpenoids in organic solvent extracts. In this study, we evaluated the biological activities of five water extracts of tissue of the mottled anemone Urticina crassicornis. Most extracts exhibited broad-spectrum antimicrobial activity as determined by ultrasensitive radial diffusion assay (URDA) against gram-positive and -negative bacteria, including a fish pathogen, Aeromonas hydrophila, but no activity against fungi. The activity of the extracts was abolished by tryptic digestion, indicating that protein compounds were responsible for the antimicrobial activity. Furthermore, in a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging activity assay, only the visceral tissue extract showed activity. However, no extract had hemolytic activity against human red blood cells. Consequently, this study suggests the water-soluble extract of mottled anemone to be a promising source of proteinaceous antimicrobial compounds that can be utilized for development of novel antibiotics.

Keyword : Mottled sea anemone, Water-soluble extract, Antimicrobial activity, Antioxidant activity, Hemolysis

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