E-FAS, vol. 18, no. 4, pp.417-420, December, 2015
Potential Association between Insulin-like Growth Factor-1
Receptor Activity and Surf Clam Spisula sachalinensis Larvae Survival
Youn Hee Choi and Taek Jeong Nam*
Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Korea
We investigated the relationship between viability and IGF-1 receptor (IGF-1R) activity in D-shaped and umbo larvae of the surf clam Spisula sachalinensis after treatment with vitrification solution (VS) or freezing. In a toxicity assay, VS1, containing 5 M dimethyl sulfoxide (DMSO), was very harmful to D-shaped and umbo larvae. However, VS2, containing 5 M ethylene glycol (EG), was not harmful to either larval stage. Although VS2 had a promising toxicity test outcome, none of the larvae survived vitrification. After immersion into VSs and freezing, IGF-1R β-subunits were detected in all larvae; however, tyrosine phosphorylation of intracellular β-subunits was detected only in the control and live groups. These results suggest that activation of IGF-1R may influence surf clam larvae viability.
Spisula sachalinensis, Surf clam, D-shaped larvae, Umbo larvae, Vitrification, IGF-1R